Evaluation of polymerase chain reaction for diagnosis of pneumococcal pneumonia

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ID: 111926
1993
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Abstract
To test the ability of the polymerase chain reaction (PCR) to detect Streptococcus pneumoniae in blood, we generated two sets of nested primers. The first defined 559-bp and 649-bp regions of the pneumolysin gene, and the second defined 445-bp and 553-bp regions of the autolysin gene. These nucleoti …
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km1993journalevaluation Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Rudolph KM;Parkinson AJ;Black CM;Mayer LW;;
Journal Journal of clinical microbiology
Year 1993
DOI
DOI not found
URL
http://www.ncbi.nlm.nih.gov/pubmed/8253962
Keywords
National Center for Biotechnology Information NCBI NLM MEDLINE humans pubmed abstract nih national institutes of health national library of medicine DNA sensitivity and specificity Polymerase Chain Reaction / methods* Base Sequence Molecular Sequence Data pneumonia pneumococcal / diagnosis* bacterial / analysis* pmid:8253962 pmc265966 doi:10.1128/jcm.31.10.2661-2666.1993 k m rudolph a j parkinson l w mayer bacteremia / diagnosis* streptococcus pneumoniae / genetics*

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