Evaluation of polymerase chain reaction for diagnosis of pneumococcal pneumonia
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ID: 111926
1993
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Abstract
To test the ability of the polymerase chain reaction (PCR) to detect Streptococcus pneumoniae in blood, we generated two sets of nested primers. The first defined 559-bp and 649-bp regions of the pneumolysin gene, and the second defined 445-bp and 553-bp regions of the autolysin gene. These nucleoti …
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| Authors | Rudolph KM;Parkinson AJ;Black CM;Mayer LW;; |
| Journal | Journal of clinical microbiology |
| Year | 1993 |
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DOI not found
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| Keywords |
National Center for Biotechnology Information
NCBI
NLM
MEDLINE
humans
pubmed abstract
nih
national institutes of health
national library of medicine
DNA
sensitivity and specificity
Polymerase Chain Reaction / methods*
Base Sequence
Molecular Sequence Data
pneumonia
pneumococcal / diagnosis*
bacterial / analysis*
pmid:8253962
pmc265966
doi:10.1128/jcm.31.10.2661-2666.1993
k m rudolph
a j parkinson
l w mayer
bacteremia / diagnosis*
streptococcus pneumoniae / genetics*
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