Selection of novel reference genes for use in the human central nervous system: a BrainNet Europe Study

Clicks: 210
ID: 111887
2012
Free PDF
Article Quality & Performance Metrics
Overall Quality
Not rated
Combines reader engagement with the AI quality analysis. This article has not been analysed, so there is no overall score — reader engagement is measured and shown alongside.
AI Quality Assessment
Not analyzed
Readership in this journal
Emerging

Ranked #123 of 438 articles by views in acta neuropathologica

Most read Least read

Bar heights use a square-root scale. Only the 120 most-read articles are drawn; the journal has 438 in total.

Mint this article as an NFT
Not yet minted

Create a permanent, verifiable on-chain record of this article on the Scimatic Network. The NFT is held in your Journament account, and you can withdraw it to your own wallet at any time.

5 SUSD one-off · no wallet required
Sign in to mint
Abstract
The use of an appropriate reference gene to ensure accurate normalisation is crucial for the correct quantification of gene expression using qPCR assays and RNA arrays. The main criterion for a gene to qualify as a reference gene is a stable expression across various cell types and experimental settings. Several reference genes are commonly in use but more and more evidence reveals variations in their expression due to the presence of on-going neuropathological disease processes, raising doubts concerning their use. We conducted an analysis of genome-wide changes of gene expression in the human central nervous system (CNS) covering several neurological disorders and regions, including the spinal cord, and were able to identify a number of novel stable reference genes. We tested the stability of expression of eight novel (ATP5E, AARS, GAPVD1, CSNK2B, XPNPEP1, OSBP, NAT5 and DCTN2) and four more commonly used (BECN1, GAPDH, QARS and TUBB) reference genes in a smaller cohort using RT-qPCR. The most stable genes out of the 12 reference genes were tested as normaliser to validate increased levels of a target gene in CNS disease. We found that in human post-mortem tissue the novel reference genes, XPNPEP1 and AARS, were efficient in replicating microarray target gene expression levels and that XPNPEP1 was more efficient as a normaliser than BECN1, which has been shown to change in expression as a consequence of neuronal cell loss. We provide herein one more suitable novel reference gene, XPNPEP1, with no current neuroinflammatory or neurodegenerative associations that can be used for gene quantitative gene expression studies with human CNS post-mortem tissue and also suggest a list of potential other candidates. These data also emphasise the importance of organ/tissue-specific stably expressed genes as reference genes for RNA studies.
Reference Key
durrenberger2012actaselection Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Pascal F. Durrenberger;Francisca S. Fernando;Roberta Magliozzi;Samira N. Kashefi;Timothy P. Bonnert;Isidro Ferrer;Danielle Seilhean;Brahim Nait-Oumesmar;Andrea Schmitt;Peter J. Gebicke-Haerter;Peter Falkai;Edna Grünblatt;Miklos Palkovits;Piero Parchi;Sabina Capellari;Thomas Arzberger;Hans Kretzschmar;Federico Roncaroli;David T. Dexter;Richard Reynolds;Pascal F. Durrenberger;Francisca S. Fernando;Roberta Magliozzi;Samira N. Kashefi;Timothy P. Bonnert;Isidro Ferrer;Danielle Seilhean;Brahim Nait-Oumesmar;Andrea Schmitt;Peter J. Gebicke-Haerter;Peter Falkai;Edna Grünblatt;Miklos Palkovits;Piero Parchi;Sabina Capellari;Thomas Arzberger;Hans Kretzschmar;Federico Roncaroli;David T. Dexter;Richard Reynolds;
Journal acta neuropathologica
Year 2012
DOI
doi:10.1007/s00401-012-1027-z
URL
https://link.springer.com/article/10.1007/s00401-012-1027-z
Keywords
pathology neurosciences

Citations

No citations found. To add a citation, contact the admin at info@scimatic.org

Comments

No comments yet. Be the first to comment on this article.