Comparison of real-time PCR with SYBR Green I or 5'-nuclease assays and dot-blot hybridization with rDNA-targeted oligonucleotide probes in quantification of selected faecal bacteria

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2003
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Abstract
PCR primers and hybridization probes were designed for the 16S rRNA genes of six bacterial species or groups typically present in human faeces or used in the dairy industry. The primers and probes were applied for quantification of the target bacterial genomes added in artificial DNA mixtures or fae …
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Authors Malinen E;Kassinen A;Rinttilä T;Palva A;;
Journal Microbiology (Reading, England)
Year 2003
DOI
DOI not found
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http://www.ncbi.nlm.nih.gov/pubmed/12576600##similar
Keywords
National Center for Biotechnology Information NCBI NLM MEDLINE RNA humans pubmed abstract nih national institutes of health national library of medicine research support non-u.s. gov't Comparative Study DNA Ribosomal 16S / genetics pmid:12576600 doi:10.1099/mic.0.25975-0 Evaluation Study Erja Malinen Anna Kassinen Airi Palva Bacteria / genetics Bacteria / isolation & purification* Bacterial / analysis Ribosomal / analysis Feces / microbiology* Fluorescent Dyes / metabolism Immunoblotting Nucleic Acid Hybridization / methods* Oligonucleotide Probes* Organic Chemicals* Polymerase Chain Reaction / methods* Taq Polymerase / metabolism

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