β-Agarase immobilized on tannic acid-modified FeO nanoparticles for efficient preparation of bioactive neoagaro-oligosaccharide.

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ID: 108432
2019
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Abstract
β-Agarase was immobilized by using tannic acid modified-FeO magnetic nanoparticles (TA-MNPs) as a supportmaterial. The MNPs were synthesized by improved chemical coprecipitation method and modified with TA for agarase immobilization. TA-MNPs and immobilized β-agarase were characterized by transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FTIR), and thermal gravimetric analysis (TGA), all of which indicated the successful surface modification of MNPs with TA and the immobilization of β-agarase. The optimal immobilization conditions for 25 mg TA-MNPs included 100 r/min oscillation speed, immobilization time of 2 h, immobilization temperature of 15 °C, and initial β-agarase concentration of 3 mL (480 U). Immobilized β-agarase showed better pH and thermal stability and excellent reusability than the free enzyme. Results revealed the promising application of β-agarase-TA-MNPs for the preparation of neoagaro-oligosaccharides with different averagepolymerizationdegrees and varying activities in the antioxidant.
Reference Key
xiao2019agarasefood Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Xiao, Qiong;Liu, Chunli;Ni, Hui;Zhu, Yanbing;Jiang, Zedong;Xiao, Anfeng;
Journal Food chemistry
Year 2019
DOI
S0308-8146(18)31406-7
URL
Keywords
Characterization immobilization neoagaro-oligosaccharides tannic acid-modified fe(3)o(4) nanoparticles β-agarase

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