Reconstruction of Single-Cell Innate Fluorescence Signatures by Confocal Microscopy.

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ID: 107915
2020
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Abstract
Described here is confocal reflection microscopy-assisted single-cell innate fluorescence analysis (CRIF), a minimally invasive method for reconstructing the innate cellular fluorescence signature from each individual live cell in a population distributed in a three-dimensional (3D) space. The innate fluorescence signature of a cell is a collection of fluorescence signals emitted by various biomolecules within the cell. Previous studies established that innate fluorescence signatures reflect various cellular properties and differences in physiological status and are a rich source of information for cell characterization and identification. Innate fluorescence signatures have been traditionally analyzed at the population level, necessitating a clonal culture, but not at the single-cell level. CRIF is particularly suitable for studies that require 3D resolution and/or selective extraction of fluorescence signals from individual cells. Because the fluorescence signature is an innate property of a cell, CRIF is also suitable for tag-free prediction of the type and/or physiological status of intact and single cells. This method may be a powerful tool for streamlined cell analysis, where the phenotype of each single cell in a heterogenous population can be directly assessed by its autofluorescence signature under a microscope without cell tagging.
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hirayama2020reconstructionjournal Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Hirayama, Tomohiro;Takabe, Kyosuke;Kiyokawa, Tatsunori;Nomura, Nobuhiko;Yawata, Yutaka;
Journal Journal of visualized experiments : JoVE
Year 2020
DOI
10.3791/61120
URL
Keywords
chemical fractionation chemical mixtures mixture toxicity remediation technologies weight-of-evidence

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