Inferring presence of the western toad (Anaxyrus boreas) species complex using environmental DNA

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ID: 107234
2018
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Abstract
Western toads (species complex comprised of Anaxyrus boreas, A. canorus, A. exsul, and A. nelsoni) are widely distributed in the western United States but are declining, particularly in the southeastern extent of their range. The subspecies A. b. boreas is listed as a Species of Greatest Conservation Need in New Mexico, Colorado, Utah, and Wyoming. Reliable and sensitive methods for delineating distributions of western toads are critical for monitoring the status of the species and prioritizing conservation efforts. We developed two qPCR assays for detecting western toad DNA in environmental DNA samples. Both markers efficiently and reliably detect low concentrations of western toad DNA across their range in the conterminous U. S. without detecting non-target, sympatric species. To determine the optimal annual sampling period, we then tested these markers using repeated sampling in ponds where western toads were known to be present. Quantities of collected eDNA varied widely across samples, but sample-level detections across sites exceeded 80% for June sampling. In the later summer, detection dropped off sharply with only a single detection in the ten samples collected throughout August. Keywords: eDNA, qPCR, Freshwater, Monitoring, Wetland, Amphibian
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franklin2018inferringglobal Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Franklin, Thomas W.;Dysthe, Joseph C.;Golden, Michael;McKelvey, Kevin S.;Hossack, Blake R.;Carim, Kellie J.;Tait, Cynthia;Young, Michael K.;Schwartz, Michael K.;
Journal global ecology and conservation
Year 2018
DOI
DOI not found
URL
http://www.sciencedirect.com/science/article/pii/S2351989418301173
Keywords
Ecology Zoology Biology (General) Medicine Science Aquaculture. Fisheries. Angling environmental effects of industries and plants renewable energy sources environmental sciences geography (general) oceanography physical geography physiology geography. anthropology. recreation

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