Generation of two LRRK2 homozygous knockout human induced pluripotent stem cell lines using CRISPR/Cas9.

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ID: 104960
2020
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Abstract
Mutations in the Leucine rich repeat kinase 2 (LRRK2) gene are found in both familial and sporadic Parkinson's disease (PD), and are also associated with immune-related disorders including Crohn's disease (CD) and leprosy. We have generated two homozygous LRRK2 knockout human induced pluripotent stem cell (iPSC) lines using CRISPR-Cas9 in a well-characterized human iPSC clone. The LRRK2 knockout cell lines retained normal morphology, gene expression, and the capacity to differentiate into cell types of the three germ layers. These cell lines are valuable for elucidating the role of LRRK2 in innate immunity and PD.
Reference Key
chen2020generationstem Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Chen, Shuhan;Luo, Zhiwei;Ward, Carl;Ibañez, David P;Liu, Hao;Zhong, Xiaofen;Sharma, Narendra K;Qin, Baoming;Fan, Wenxia;Wang, Dongye;
Journal Stem cell research
Year 2020
DOI
S1873-5061(20)30106-9
URL
Keywords
metastasis neuroblastoma lin28b let-7 pdz binding kinase

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