Impact of deoxycholate on growth, toxin production, and sporulation.

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ID: 104592
2020
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Abstract
Bile acids play an important role in life cycle. Deoxycholate (DCA), one of the most abundant secondary bile acids, is known to inhibit vegetative growth and toxin production. However, limited data are available on the role of DCA on sporulation. Here, we investigated the phenotypic and genotypic impact of DCA on the growth, toxin production, and sporulation of .Four genetically divergent strains were cultured in nutrient-rich broth with and without DCA at various concentrations, and growth activity was evaluated for each strain. Cytotoxicity assays using culture supernatants from cells grown in nutrient-rich broth with and without 0.01% DCA were conducted. Sporulation efficiency was determined using sporulation media with and without 0.01% DCA. Transcript levels of and were analyzed using quantitative reverse-transcription polymerase chain reaction.We found that DCA led to growth reduction in a dose-depended manner and regulated toxin production by repressing expression during vegetative growth. To our knowledge, we have also provided the first evidence that DCA reduces sporulation efficiency through the downregulation of expression during the sporulation stage.DCA modulates sporulation, vegetative growth, and toxin production.
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usui2020impactheliyon Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Usui, Yukino;Ayibieke, Alafate;Kamiichi, Yuko;Okugawa, Shu;Moriya, Kyoji;Tohda, Shuji;Saito, Ryoichi;
Journal Heliyon
Year 2020
DOI
10.1016/j.heliyon.2020.e03717
URL
Keywords
Microbiology clostridioides difficile infectious disease sporulation bacteriology deoxycholate hepatobiliary system medical microbiology microbiology epidemiology toxin production vegetative growth

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