Mathematical Approaches in Estimating Aptamer Target Binding Affinity.

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ID: 104467
2020
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Abstract
The performance of aptamers as versatile biomolecular tools in numerous analytical applications is critically dependent on their high target binding specificity and selectivity. However, only the technical or methodological aspects of measuring aptamer-target binding affinities are focused, ignoring the equally important mathematical components that play pivotal roles in affinity measurements. Hence through this paper, we aim to provide a comprehensive review regarding the utilization of different mathematical models and equations, along with a detailed description of the computational steps involved in mathematically deriving the binding affinity of aptamers against their specific target molecules. Mathematical models ranging from one-site binding to multiple aptameric binding site-based models are explained in detail. Models applied in several different approaches of affinity measurements such as thermodynamics and kinetic analysis, including cooperativity and competitive-assay based mathematical models have been elaborately discussed. Mathematical models incorporating factors that could potentially affect affinity measurements are also further scrutinized.
Reference Key
thevendran2020mathematicalanalytical Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Thevendran, Ramesh;Navien, Tholasi Nadhan;Meng, Xin;Wen, Kechun;Lin, Qiao;Sarah, Shigdar;Tang, Thean-Hock;Citartan, Marimuthu;
Journal analytical biochemistry
Year 2020
DOI
S0003-2697(20)30274-8
URL
Keywords
binding affinity mathematical models aptamers-target interactions k(d) values binding curve mathematical approaches

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