Assessment of exopolysaccharide production by subsp. ropy strain in different substrate media.

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ID: 101259
2020
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Abstract
The aim of this research was to determine the optimal medium for Exopolysaccharides (EPS) production by a subsp. ropy strain isolated from a locally produced commercial fermented milk, in reconstituted skim milk (RSM) 10% (w/v), milk whey (MW), and soy milk whey (SMW), under optimal growth conditions for this strain. Milk whey was made by coagulating fresh milk using papaya latex 3% (v/v); soy milk whey was obtained from tofu household industry. The chemical composition of the substrate media was determined by proximate analysis, and sterilization was accomplished in an autoclave at 121°C for 15 min. Culture media were inoculated with 1% (v/v) of a starter culture of subsp and then incubated at 30°C for 16 hr. EPS production, lactic acid content, cell counting, and pH were determined after the media were cooled at 5°C. Findings showed that on the basis of the growth characteristics of subsp, the best medium for EPS production was RSM 10% (258.60 ± 26.86 mg/L) compared to the milk whey (69.60 ± 9.48 mg/L) and soy milk whey (49.80 ± 9.04 mg/L).
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malaka2020assessmentfood Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Malaka, Ratmawati;Maruddin, Fatma;Dwyana, Zaraswati;Vargas, Maynor V;
Journal Food science & nutrition
Year 2020
DOI
10.1002/fsn3.1452
URL
Keywords
exopolysaccharide lactobacillus delbrueckii subsp. bulgaricus reconstituted skim milk ropy strain soy milk whey

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