Detection of Glycoproteins in Polyacrylamide Gels Using Pro-Q Emerald 300 Dye, a Fluorescent Periodate Schiff-Base Stain.
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ID: 100192
2018
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Abstract
Pro-Q Emerald 300 glycoprotein stain generates a bright-green fluorescent signal upon reacting with periodic acid-oxidized carbohydrate groups on proteins. With this dye it is possible to detect proteins directly in the gel without the need to transfer them to a membrane. This dye is more sensitive than the standard periodic-acid Schiff's base which uses acidic fuchsin dye.
| Reference Key |
mehtadsouza2018detectionmethods
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| Authors | Mehta-D'souza, Padmaja; |
| Journal | methods in molecular biology (clifton, nj) |
| Year | 2018 |
| DOI |
10.1007/978-1-4939-8745-0_14
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