RNA Extraction from Equine Samples for Equine Influenza Virus.

Clicks: 245
ID: 100156
2020
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Ranked #86 of 139 articles by views in methods in molecular biology (clifton, nj)

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Abstract
The primary goals of this chapter are to discuss common viral RNA isolation and purification methods that are routinely used by various diagnostic laboratories and to highlight the advantages and drawbacks of each method and to identify the most suitable and reliable method to increase the sensitivity and specificity of RT-PCR assays for the detection of equine influenza virus (EIV) in clinical specimens. Our experiences and review of literature show that magnetic bead-based nucleic extraction methods (manual and automatic) work well for isolation and purification of EIV RNA from nasal swab specimens. Furthermore, most of the information presented in this chapter could be directly applicable to isolation and purification of nucleic acids (both DNA and RNA) from other equine clinical samples.
Reference Key
balasuriya2020rnamethods Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Balasuriya, Udeni B R;
Journal methods in molecular biology (clifton, nj)
Year 2020
DOI
10.1007/978-1-0716-0346-8_28
URL
Keywords
equine influenza virus magnetic beads rna purification viral rna

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