cytotoxic evaluation of e-liquid aerosol using different lung-derived cell models

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ID: 201266
2015
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摘要
The in vitro toxicological evaluation of e-liquid aerosol is an important aspect of consumer protection, but the cell model is of great significance. Due to its water solubility, e-liquid aerosol is deposited in the conducting zone of the respiratory tract. Therefore, primary normal human bronchial epithelial (NHBE) cells are more suitable for e-liquid aerosol testing than the widely used alveolar cell line A549. Due to their prolonged lifespan, immortalized cell lines derived from primary NHBE cells, exhibiting a comparable in vitro differentiation, might be an alternative for acute toxicity testing. In our study, A549 cells freshly isolated NHBE cells and the immortalized cell line CL-1548 were exposed at the air-liquid interface to e-liquid aerosol and cigarette mainstream smoke in a CULTEX® RFS compact module. The cell viability was analyzed 24 h post-exposure. In comparison with primary NHBE cells, the CL-1548 cell line showed lower sensitivity to e-liquid aerosol but significantly higher sensitivity compared to A549 cells. Therefore, the immortalized cell line CL-1548 is recommended as a tool for the routine testing of e-liquid aerosol and is preferable to A549 cells.
参考键
scheffler2015internationalcytotoxic 使用此键在稿件中自动引用，同时使用 SciMatic 稿件管理器或论文管理器
作者 ;Stefanie Scheffler;Hauke Dieken;Olaf Krischenowski;Michaela Aufderheide
期刊 archives of biochemistry and biophysics
年份 2015
DOI
10.3390/ijerph121012466
URL
http://www.mdpi.com/1660-4601/12/10/12466
关键词
Cytotoxicity electronic cigarette smoking tobacco nicotine

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