Intestinal Preservation Injury: A Comparison Between Rat, Porcine and Human Intestines.

Clicks: 331
ID: 97992
2019
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Abstract
Advanced preservation injury (PI) after intestinal transplantation has deleterious short- and long-term effects and constitutes a major research topic. Logistics and costs favor rodent studies, whereas clinical translation mandates studies in larger animals or using human material. Despite diverging reports, no direct comparison between the development of intestinal PI in rats, pigs, and humans is available. We compared the development of PI in rat, porcine, and human intestines. Intestinal procurement and cold storage (CS) using histidine-tryptophan-ketoglutarate solution was performed in rats, pigs, and humans. Tissue samples were obtained after 8, 14, and 24 h of CS), and PI was assessed morphologically and at the molecular level (cleaved caspase-3, zonula occludens, claudin-3 and 4, tricellulin, occludin, cytokeratin-8) using immunohistochemistry and Western blot. Intestinal PI developed slower in pigs compared to rats and humans. Tissue injury and apoptosis were significantly higher in rats. Tight junction proteins showed quantitative and qualitative changes differing between species. Significant interspecies differences exist between rats, pigs, and humans regarding intestinal PI progression at tissue and molecular levels. These differences should be taken into account both with regards to study design and the interpretation of findings when relating them to the clinical setting.
Reference Key
sfteland2019intestinalinternational Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Søfteland, John Mackay;Casselbrant, Anna;Biglarnia, Ali-Reza;Linders, Johan;Hellström, Mats;Pesce, Antonio;Padma, Arvind Manikantan;Jiga, Lucian Petru;Hoinoiu, Bogdan;Ionac, Mihai;Oltean, Mihai;
Journal International journal of molecular sciences
Year 2019
DOI
E3135
URL
Keywords
organ preservation transplantation ischemia tight junctions intestine intestinal mucosa

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