Localization of Tricellular Tight Junction Molecule LSR at Midbody and Centrosome During Cytokinesis in Human Epithelial Cells.

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ID: 97984
2020
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Abstract
Epithelial integrity and barrier function are maintained during cytokinesis in vertebrate epithelial tissues. The changes in localization and the roles of tricellular tight junction molecule lipolysis-stimulated lipoprotein receptor (LSR) during cytokinesis are not well known, although new tricellular tight junctions form at the flank of the midbody during cytokinesis. In this study, we investigated the changes in localization and the role of LSR at the midbody and centrosome during cytokinesis using human endometrial carcinoma cell line Sawano, comparing the tricellular tight junction molecule tricellulin; bicellular tight junction molecules occludin, claudin-7, zonula occludens-1, and cingulin; and the epithelial polarized related molecules apoptosis-stimulating of p53 protein 2, PAR3, and yes-associated protein. During cytokinesis induced by treatment with taxol, the epithelial barrier was maintained and the tricellular tight junction molecules LSR and tricellulin were concentrated at the flank of the acetylated tubulin-positive midbody and in γ-tubulin-positive centrosomes with the dynein adaptor Hook2, whereas the other molecules were localized there as well. All the molecules disappeared by knockdown using small interfering RNAs. Furthermore, by the knockdown of Hook2, the epithelial barrier was maintained and most of the molecules disappeared from the centrosome. These findings suggest that LSR may play crucial roles not only in barrier function but also in cytokinesis.
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konno2020localizationthe Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Konno, Takumi;Kohno, Takayuki;Kikuchi, Shin;Shimada, Hiroshi;Satohisa, Seiro;Takano, Kenichi;Saito, Tsuyoshi;Kojima, Takashi;
Journal The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society
Year 2020
DOI
10.1369/0022155419886263
URL
Keywords
yap centrosome tricellulin aspp2 hook2 lsr bicellular tight junctions cytokinesis midbody tricellular tight junctions

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