Footprints of a trypanosomatid RNA world: pre-small subunit rRNA processing by spliced leader addition trans-splicing

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ID: 97940
2012
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Abstract
The addition of a capped mini-exon [spliced leader (SL)] through trans-splicing is essential for the maturation of RNA polymerase (pol) II-transcribed polycistronic pre-mRNAs in all members of the Trypanosomatidae family. This process is an inter-molecular splicing reaction that follows the same basic rules of cis-splicing reactions. In this study, we demonstrated that mini-exons were added to precursor ribosomal RNA (pre-rRNA) are transcribed by RNA pol I, including the 5' external transcribed spacer (ETS) region. Additionally, we detected the SL-5'ETS molecule using three distinct methods and located the acceptor site between two known 5'ETS rRNA processing sites (A' and A1) in four different trypanosomatids. Moreover, we detected a polyadenylated 5'ETS upstream of the trans-splicing acceptor site, which also occurs in pre-mRNA trans-splicing. After treatment with an indirect trans-splicing inhibitor (sinefungin), we observed SL-5'ETS decay. However, treatment with 5-fluorouracil (a precursor of RNA synthesis that inhibits the degradation of pre-rRNA) led to the accumulation of SL-5'ETS, suggesting that the molecule may play a role in rRNA degradation. The detection of trans-splicing in these molecules may indicate broad RNA-joining properties, regardless of the polymerase used for transcription.
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mayer2012footprintsmemorias Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Mayer, Mario Gustavo;Santos, Marcos Gonzaga dos;Silva, Maria Fernanda Laranjeira da;Floeter-Winter, Lucile Maria;
Journal memórias do instituto oswaldo cruz
Year 2012
DOI
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URL
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762012000400013&lng=en&tlng=en
Keywords
Microbiology Biology (General) Medicine (General) Medicine Immunologic diseases. Allergy public aspects of medicine arctic medicine. tropical medicine pathology

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