Nuclear export of replication protein A in the non-replicative infective forms of Trypanosoma cruzi.

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ID: 97903
2020
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Abstract
Replication Protein A (RPA), a heterotrimeric complex, is the major single-stranded DNA-binding protein in eukaryotes. Recently, we characterized RPA from Trypanosoma cruzi, showing that it is involved in DNA replication and DNA damage response in this organism. Better efficiency in differentiation from epimastigote to metacyclic trypomastigote forms was observed in TcRPA-2 subunit heterozygous knockout cells, suggesting that RPA is involved in this process. Here, we show that RPA cellular localization changes during the T. cruzi life cycle, with RPA being detected only in the cytoplasm of the metacyclic and bloodstream trypomastigotes. We also identify a Nuclear Export Signal (NES) in the trypanosomatid RPA-2 subunit. Mutations in the negatively charged residues of RPA-2 NES impair the differentiation process, suggesting that RPA exportation affects parasite differentiation into infective forms.
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pavani2020nuclearfebs Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Pavani, Raphael S;de Lima, Loyze P;Lima, André A;Fernandes, Carlos A H;Fragoso, Stenio P;Calderano, Simone G;Elias, Maria Carolina;
Journal FEBS Letters
Year 2020
DOI
10.1002/1873-3468.13755
URL
Keywords
t. cruzi nes nuclear exportation rpa replication protein a

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