Two ground state isoforms and a chromophore D-ring photoflip triggering extensive intramolecular changes in a canonical phytochrome.

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ID: 97345
2011
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Ranked #46 of 292 articles by views in Proceedings of the National Academy of Sciences of the United States of America

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Abstract
Phytochrome photoreceptors mediate light responses in plants and in many microorganisms. Here we report studies using (1)H-(13)C magic-angle spinning NMR spectroscopy of the sensor module of cyanobacterial phytochrome Cph1. Two isoforms of the red-light absorbing Pr ground state are identified. Conclusive evidence that photoisomerization occurs at the C15-methine bridge leading to a β-facial disposition of the ring D is presented. In the far-red-light absorbing Pfr state, strong hydrogen-bonding interactions of the D-ring carbonyl group to Tyr-263 and of N24 to Asp-207 hold the chromophore in a tensed conformation. Signaling is triggered when Asp-207 is released from its salt bridge to Arg-472, probably inducing conformational changes in the tongue region. A second signal route is initiated by partner swapping of the B-ring propionate between Arg-254 and Arg-222.
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song2011twoproceedings Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Song, Chen;Psakis, Georgios;Lang, Christina;Mailliet, Jo;Gärtner, Wolfgang;Hughes, Jon;Matysik, Jörg;
Journal Proceedings of the National Academy of Sciences of the United States of America
Year 2011
DOI
10.1073/pnas.1013377108
URL
Keywords
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