Ten antenna proteins are associated with the core in the supramolecular organization of the photosystem I supercomplex in .

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2019
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Abstract
Photosystem I (PSI) is a large pigment-protein complex mediating light-driven charge separation and generating a highly negative redox potential, which is eventually utilized to produce organic matter. In plants and algae, PSI possesses outer antennae, termed light-harvesting complex I (LHCI), which increase the energy flux to the reaction center. The number of outer antennae for PSI in the green alga is known to be larger than that of land plants. However, their exact number and location remain to be elucidated. Here, applying a newly established sample purification procedure, we isolated a highly pure PSI-LHCI supercomplex containing all nine gene products under state 1 conditions. Single-particle cryo-EM revealed the 3D structure of this supercomplex at 6.9 Å resolution, in which the densities near the PsaF and PsaJ subunits were assigned to two layers of LHCI belts containing eight LHCIs, whereas the densities between the PsaG and PsaH subunits on the opposite side of the LHCI belt were assigned to two extra LHCIs. Using single-particle cryo-EM, we also determined the 2D projection map of the mutant, which confirmed the assignment of LHCA2 and LHCA9 to the densities between PsaG and PsaH. Spectroscopic measurements of the PSI-LHCI supercomplex suggested that the bound LHCA2 and LHCA9 proteins have the ability to increase the light-harvesting energy for PSI. We conclude that the PSI in has a larger and more distinct outer-antenna organization and higher light-harvesting capability than that in land plants.
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kubotakawai2019tenthe Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Kubota-Kawai, Hisako;Burton-Smith, Raymond N;Tokutsu, Ryutaro;Song, Chihong;Akimoto, Seiji;Yokono, Makio;Ueno, Yoshifumi;Kim, Eunchul;Watanabe, Akimasa;Murata, Kazuyoshi;Minagawa, Jun;
Journal The Journal of biological chemistry
Year 2019
DOI
10.1074/jbc.RA118.006536
URL
Keywords
photosynthesis cryo-electron microscopy photosystem i chlamydomonas reinhardtii chlamydomonas lhci cryo-electron microscopy (cryo-em) green algae light-harvesting complex (antenna complex) outer antenna photosynthetic efficiency single-particle analysis

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