Rewritable multi-event analog recording in bacterial and mammalian cells.

Clicks: 264
ID: 96157
2018
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Abstract
We present two CRISPR-mediated analog multi-event recording apparatus (CAMERA) systems that use base editors and Cas9 nucleases to record cellular events in bacteria and mammalian cells. The devices record signal amplitude or duration as changes in the ratio of mutually exclusive DNA sequences (CAMERA 1) or as single-base modifications (CAMERA 2). We achieved recording of multiple stimuli in bacteria or mammalian cells, including exposure to antibiotics, nutrients, viruses, light, and changes in Wnt signaling. When recording to multicopy plasmids, reliable readout requires as few as 10 to 100 cells. The order of stimuli can be recorded through an overlapping guide RNA design, and memories can be erased and re-recorded over multiple cycles. CAMERA systems serve as "cell data recorders" that write a history of endogenous or exogenous signaling events into permanent DNA sequence modifications in living cells.
Reference Key
tang2018rewritablescience Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Tang, Weixin;Liu, David R;
Journal science (new york, ny)
Year 2018
DOI
eaap8992
URL
Keywords
edge detection analog-to-digital converter cmos image sensor xor iris recognition

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