Simplified determination of the content and average degree of acetylation of chitin in crude black soldier fly larvae samples.

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ID: 94068
2020
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Abstract
Insects are considered a promising alternative protein source for food and feed, but contain significant amounts of chitin, often undesirable due to indigestibility, disagreeable texture and negative effect on nutrients intake. Fractionation strategies are thus increasingly being applied to isolate and valorize chitin separately. The analysis of chitin generally requires an intensive pretreatment to remove impurities, and derivatization to generate sufficient detector response. In this work, a liquid chromatography method, without pretreatment nor derivatization, was developed for the simultaneous determination of chitin content and degree of acetylation in non-purified samples of black soldier fly (BSF) larvae. The method is found to be more suitable, compared to traditional methods, for assessing high degrees of acetylation. For the first time, the degree of acetylation of BSF chitin (81 ± 2%) is reported. Additionally, the chitin content of BSF soft tissues is estimated at approximately 20% of the total chitin content (8.5 ± 0.1%).
Reference Key
dhondt2020simplifiedcarbohydrate Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors D'Hondt, Els;Soetemans, Lise;Bastiaens, Leen;Maesen, Miranda;Jespers, Vincent;Van den Bosch, Bert;Voorspoels, Stefan;Elst, Kathy;
Journal Carbohydrate research
Year 2020
DOI
S0008-6215(19)30319-2
URL
Keywords
analysis black soldier fly larvae chitin content crude samples degree of acetylation fast method uplc-ms

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