A Fast and Effective Microfluidic Spraying-Plunging Method for High-Resolution Single-Particle Cryo-EM.

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ID: 92247
2017
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Abstract
We describe a spraying-plunging method for preparing cryoelectron microscopy (cryo-EM) grids with vitreous ice of controllable, highly consistent thickness using a microfluidic device. The new polydimethylsiloxane (PDMS)-based sprayer was tested with apoferritin. We demonstrate that the structure can be solved to high resolution with this method of sample preparation. Besides replacing the conventional pipetting-blotting-plunging method, one of many potential applications of the new sprayer is in time-resolved cryo-EM, as part of a PDMS-based microfluidic reaction channel to study short-lived intermediates on the timescale of 10-1,000 ms.
Reference Key
feng2017astructure Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Feng, Xiangsong;Fu, Ziao;Kaledhonkar, Sandip;Jia, Yuan;Shah, Binita;Jin, Amy;Liu, Zheng;Sun, Ming;Chen, Bo;Grassucci, Robert A;Ren, Yukun;Jiang, Hongyuan;Frank, Joachim;Lin, Qiao;
Journal structure (london, england : 1993)
Year 2017
DOI
S0969-2126(17)30036-9
URL
Keywords
apoferritin em grid preparation pdms-based microsprayer ice thickness time-resolved cryo-em

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