Liquid Phase and Microwave-Assisted Extractions for Multicomponent Phenolic Pattern Determination of Five Romanian Galium Species Coupled with Bioassays.
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2019
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Abstract
: is a plant rich in iridoid glycosides, flavonoids, anthraquinones, and small amounts of essential oils and vitamin C. Recent works showed the antibacterial, antifungal, antiparasitic, and antioxidant activity of this plant genus. : For the determination of the multicomponent phenolic pattern, liquid phase microextraction procedures were applied, combined with HPLC-PDA instrument configuration in five species aerial parts (, , , , and ). Dispersive Liquid⁻Liquid MicroExtraction (DLLME) with NaCl and NAtural Deep Eutectic Solvent (NADES) medium and Ultrasound-Assisted (UA)-DLLME with β-cyclodextrin medium were optimized. : The optimal DLLME conditions were found to be: 10 mg of the sample, 10% NaCl, 15% NADES or 1% β-cyclodextrin as extraction solvent-400 μL of ethyl acetate as dispersive solvent-300 μL of ethanol, vortex time-30 s, extraction time-1 min, centrifugation at 12000× for 5 min. : These results were compared with microwave-assisted extraction procedures. and extracts showed the highest total phenolic and flavonoid content, respectively. The most potent extract in terms of antioxidant capacity was obtained from , whereas the extract obtained from exhibited the strongest inhibitory effect against tyrosinase.
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mocan2019liquidmolecules
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| Authors | Mocan, Andrei;Diuzheva, Alina;Bădărău, Sabin;Moldovan, Cadmiel;Andruch, Vasil;Carradori, Simone;Campestre, Cristina;Tartaglia, Angela;De Simone, Marta;Vodnar, Dan;Tiecco, Matteo;Germani, Raimondo;Crișan, Gianina;Locatelli, Marcello; |
| Journal | molecules |
| Year | 2019 |
| DOI |
E1226
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| URL | |
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