Presence of Heavy Metal Resistance Genes in Escherichia coli and Salmonella, and Analysis of Resistance Gene Structure in E. coli E308.

Clicks: 346
ID: 90674
2020
Article Quality & Performance Metrics
Overall Quality
Not rated
Combines reader engagement with the AI quality analysis. This article has not been analysed, so there is no overall score — reader engagement is measured and shown alongside.
AI Quality Assessment
Not analyzed
Readership in this journal
Emerging

Ranked #12 of 113 articles by views in Journal of global antimicrobial resistance

Most read Least read

Bar heights use a square-root scale.

Mint this article as an NFT
Not yet minted

Create a permanent, verifiable on-chain record of this article on the Scimatic Network. The NFT is held in your Journament account, and you can withdraw it to your own wallet at any time.

5 SUSD one-off · no wallet required
Sign in to mint
Abstract
Little attention has been paid to heavy metal resistance (HMR) to pathogenic bacteria with the wide use of heavy metals as feed additives in food animals. Therefore, present study was constructed to investigate the presence of HMR in Escherichia coli and Salmonella, and its correlation with disinfectant resistance genes (DRGs) and antibiotic resistance genes (ARGs).The heavy metal resistance genes (HMRGs) of 178 E. coli and 294 Salmonella isolated from chicken farms and retail meats were detected by polymerase chain reaction (PCR). The minimal inhibitory concentrations (MICs) of heavy metals were determined by broth micro-dilution method. The complete-genome of E. coli E308 which had indications of multi-resistance was recovered and assembled using third-generation sequencing.Results showed that frequency of different HMRGs in E. coli and Salmonella ranged 0.60 - 77.0% and 0.30 - 87.1%, respectively. MICs of heavy metals for E. coli and Salmonella ranged widely from ≤ 12.5 mg/L to 1600 mg/L. Moreover, the HMRGs (zntA, arsB, merA, pcoR, pcoA, pcoC and chrA) were found to be significantly associated with one or more DRGs (sugE(c), emrE, mdfA, ydgE/ydgF, qacF, sugE(p) and qacEΔ1) and ARGs (sul1, sul2, sul3, tetA, tetB, tetC, bla, bla and bla) (p < 0.05).The present study demonstrated that HMRGs were widely present in E. coli and Salmonella isolated from chicken farm and retail meats, and the association between HMRGs with DRGs and ARGs may lead to the co-resistance of heavy metals and other antimicrobials.
Reference Key
yang2020presencejournal Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Yang, Shengzhi;Deng, Wenwen;Liu, Shuliang;Yu, Xiumei;Mustafa, Ghulam Raza;Chen, Shujuan;He, Li;Ao, Xiaoling;Yang, Yong;Zhou, Kang;Li, Bei;Han, Xinfeng;Xu, Xuebin;Zou, Likou;
Journal Journal of global antimicrobial resistance
Year 2020
DOI
S2213-7165(20)30010-2
URL
Keywords
Escherichia coli salmonella whole-genome sequencing antibiotic and disinfectant resistance heavy metal resistance

Citations

No citations found. To add a citation, contact the admin at info@scimatic.org

Comments

No comments yet. Be the first to comment on this article.