Sensitive colorimetric determination of microRNA let-7a through rolling circle amplification and a peroxidase-mimicking system composed of trimeric G-triplex and hemin DNAzyme.

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ID: 90590
2020
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Abstract
The authors have incidentally found that the three tandem repeats of a 13-mer G-rich oligomer (with sequence 5'-TGG GAA GGG AGG G-3'; referred to as G3) can directly fold into a stable G3 trimer. The G3 trimer/hemin DNAzyme exhibits an about 3-fold higher peroxidase-mimicking activity compared to the conventional G3/hemin DNAzyme. Combining this finding with rolling circle amplification (RCA), a colorimetric assay was developed for sensitive and specific determination of microRNA. In this method, each cycle of RCA generates three catalytic units. This leads to a significant signal amplification of the RCA. Using let-7a as a model analyte, the colorimetric method (best performed at 420 nm) exhibits high sensitivity toward microRNA-let-7a with a 37 fM detection limit and an analytical range that covers 3 orders of magnitude. The method was applied to the determination of let-7a in some cell lysates. Graphical abstractThis G-triplex trimer-based rolling circle amplification (RCA) method can produce three catalytic units per RCA cycle, which can significantly improve the amplification efficiency of RCA.
Reference Key
li2020sensitivemikrochimica Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Li, Ruiying;Liu, Qiang;Jin, Yan;Li, Baoxin;
Journal Mikrochimica acta
Year 2020
DOI
10.1007/s00604-019-4093-2
URL
Keywords
H2O2 abts dnazyme isothermal amplification multiple g-triplex

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