Transcriptome Modifications in the Porcine Intramuscular Adipocytes during Differentiation and Exogenous Stimulation with TNF-α and Serotonin.

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2020
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Abstract
Adipocytes are dynamic cells that have critical functions to maintain body energy homeostasis. Adipocyte physiology is affected by the adipogenic differentiation, cell program, as well as by the exogenous stimulation of biochemical factors, such as serotonin and TNF-α. In this work, we investigated the global transcriptome modifications when porcine intramuscular preadipocyte (PIP) was differentiated into porcine mature adipocyte (pMA). Moreover, we studied transcriptome changes in pMA after stimulation with serotonin or TNF-α by using a microarray approach. Transcriptome analysis revealed that the expression of 270, 261, and 249 genes were modified after differentiation, or after serotonin and TNF-α stimulation, respectively. Expression changes in , , , , , , , , , , , , , , , , , , and genes which are enriched in the 'PPAR signaling' and 'insulin resistance' pathways were found in adipocytes during the differentiation process. Dose-dependent serotonin stimulation resulted in a decreased fat accumulation in pMAs. Serotonin-induced differentially expressed genes in pMAs were found to be involved in the significant enrichment of 'GPCR ligand-binding', 'cell chemotaxis', 'blood coagulation and complement', 'metabolism of lipid and lipoproteins', 'regulation of lipid metabolism by , and 'lipid digestion, mobilization and transport' pathways. TNF-α stimulation also resulted in transcriptome modifications linked with proinflammatory responses in the pMA of intramuscular origin. Our results provide a landscape of transcriptome modifications and their linked-biological pathways in response to adipogenesis, and exogenous stimulation of serotonin- and TNF-α to the pMA of intramuscular origin.
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tada2020transcriptomeinternational Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Tada, Asuka;Islam, Md Aminul;Kober, Akm Humayun;Fukuyama, Kohtaro;Takagi, Michihiro;Igata, Manami;Albarracin, Leonardo;Ikeda-Ohtsubo, Wakako;Miyazawa, Kenji;Yoda, Kazutoyo;He, Fang;Takahashi, Hideki;Villena, Julio;Aso, Hisashi;Kitazawa, Haruki;
Journal International journal of molecular sciences
Year 2020
DOI
E638
URL
Keywords
TNF-α transcriptome serotonin adipogenesis porcine intramuscular preadipocyte (pip) porcine mature adipocyte (pma) serotonin receptor fatigue life low-cycle fatigue al-9si-4cu-0.4mg-0.3sc alloy deformation mechanism different casting state

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