Correlation of Anti- Antibodies Between Serum and Saliva Samples Collected From Finisher Pigs.

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ID: 88029
2019
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Abstract
Saliva samples obtained by using absorptive devices, can provide an alternative diagnostic matrix to serum for monitoring disease status in pigs. The aim of this study was to investigate the correlation of anti- antibodies between serum and saliva samples collected from pigs. Twenty individual paired serum and saliva samples were collected from a single farm. Anti- IgG was detected in individual serum samples using a commercial ELISA kit, validated for sera. The same kit was used with a protocol modified by extending incubation time and increasing temperature to test individual saliva samples. Anti- IgG antibodies in pig saliva were always detected at a lower level than in the matching serum samples. A correlation ( = 0.66; = 0.002) and a moderate agreement ( > 0.62 = 0.003) was found between individual IgG in serum and saliva samples. Both correlation and the agreement levels are moderate. The size of this investigation was small, and further studies are necessary to further confirm these findings. The results of this work provide some evidence that saliva samples have the potential to be used for the diagnosis of infection in pig farms.
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de-lucia2019correlationfrontiers Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors De Lucia, Alessia;Cawthraw, Shaun;Davies, Rob;Smith, Richard P;Bianco, Carlo;Ostanello, Fabio;Martelli, Francesca;
Journal Frontiers in veterinary science
Year 2019
DOI
10.3389/fvets.2019.00489
URL
Keywords
ELISA serum oral fluid pigs saliva salmonella antibody

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