Loop-mediated Isothermal Amplification Assay to Rapidly Detect Wheat Streak Mosaic Virus in Quarantined Plants

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ID: 86617
2015
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Abstract
We developed a loop-mediated isothermal amplification (LAMP) method to rapidly diagnose Wheat streak mosaic virus (WSMV) during quarantine inspections of imported wheat, corn, oats, and millet. The LAMP method was developed as a plant quarantine inspection method for the first time, and its simplicity, quickness, specificity and sensitivity were verified compared to current reverse transcription-polymerase chain reaction (RT-PCR) and nested PCR quarantine methods. We were able to quickly screen for WSMV at quarantine sites with many test samples; thus, this method is expected to contribute to plant quarantine inspections.
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Authors Lee, Siwon;Kim, Jin-Ho;Choi, Ji-Young;Jang, Won-Cheoul;
Journal The plant pathology journal
Year 2015
DOI
DOI not found
URL
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4677754
Keywords
Information technology Agriculture (General) environmental effects of industries and plants renewable energy sources environmental sciences nutrition. foods and food supply plant culture political science political science (general) agricultural industries

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