Reference gene selection for quantitative real-time PCR analysis in virus infected cells: SARS corona virus, Yellow fever virus, Human Herpesvirus-6, Camelpox virus and Cytomegalovirus infections

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ID: 86393
2005
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Abstract

Abstract

Ten potential reference genes were compared for their use in experiments investigating cellular mRNA expression of virus infected cells. Human cell lines were infected with Cytomegalovirus, Human Herpesvirus-6, Camelpox virus, SARS coronavirus or Yellow fever virus. The expression levels of these genes and the viral replication were determined by real-time PCR. Genes were ranked by the BestKeeper tool, the GeNorm tool and by criteria we reported previously. Ranking lists of the genes tested were tool dependent. However, over all, β-actin is an unsuitable as reference gene, whereas TATA-Box binding protein and peptidyl-prolyl-isomerase A are stable reference genes for expression studies in virus infected cells.

Reference Key
a2005referencevirology Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors A, Müller Marcel;Hi-Gung, Bae;Stefanie, Thulke;Aleksandar, Radonić;Wolfgang, Siegert;Andreas, Nitsche;
Journal virology journal
Year 2005
DOI
DOI not found
URL
http://www.virologyj.com/content/2/1/7
Keywords
Microbiology Therapeutics. Pharmacology Infectious and parasitic diseases Medicine Technology Science public aspects of medicine anesthesiology

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