GATA3 Promotes the Neural Progenitor State but Not Neurogenesis in 3D Traumatic Injury Model of Primary Human Cortical Astrocytes

Clicks: 307
ID: 84233
2019
Free PDF
Article Quality & Performance Metrics
Overall Quality Improving Quality
0.0 /100
Combines engagement data with AI-assessed academic quality
AI Quality Assessment
Not analyzed
Abstract
Astrocytes are abundant cell types in the vertebrate central nervous system and can act as neural stem cells in specialized niches where they constitutively generate new neurons. Outside the stem cell niches, however, these glial cells are not neurogenic. Although injuries in the mammalian central nervous system lead to profound proliferation of astrocytes, which cluster at the lesion site to form a gliotic scar, neurogenesis does not take place. Therefore, a plausible regenerative therapeutic option is to coax the endogenous reactive astrocytes to a pre-neurogenic progenitor state and use them as an endogenous reservoir for repair. However, little is known on the mechanisms that promote the neural progenitor state after injuries in humans. Gata3 was previously found to be a mechanism that zebrafish brain uses to injury-dependent induction of neural progenitors. However, the effects of GATA3 in human astrocytes after injury are not known. Therefore, in this report, we investigated how overexpression of GATA3 in primary human astrocytes would affect the neurogenic potential before and after injury in 2D and 3D cultures. We found that primary human astrocytes are unable to induce GATA3 after injury. Lentivirus-mediated overexpression of GATA3 significantly increased the number of GFAP/SOX2 double positive astrocytes and expression of pro-neural factor ASCL1, but failed to induce neurogenesis, suggesting that GATA3 is required for enhancing the neurogenic potential of primary human astrocytes and is not sufficient to induce neurogenesis alone.
Reference Key
celikkaya2019gata3frontiers Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Celikkaya, Hilal;Celikkaya, Hilal;Cosacak, Mehmet Ilyas;Papadimitriou, Christos;Popova, Stanislava;Popova, Stanislava;Bhattarai, Prabesh;Biswas, Srijeeta Nag;Biswas, Srijeeta Nag;Siddiqui, Tohid;Wistorf, Sabrina;Nevado-Alcalde, Isabel;Naumann, Lisa;Mashkaryan, Violeta;Mashkaryan, Violeta;Brandt, Kerstin;Freudenberg, Uwe;Freudenberg, Uwe;Werner, Carsten;Werner, Carsten;Kizil, Caghan;Kizil, Caghan;
Journal frontiers in cellular neuroscience
Year 2019
DOI
DOI not found
URL
https://www.frontiersin.org/article/10.3389/fncel.2019.00023/full
Keywords
chemistry Organic Chemistry Education Biology (General) Botany Medicine Materials of engineering and construction. Mechanics of materials Diseases of the circulatory (Cardiovascular) system neurosciences. biological psychiatry. neuropsychiatry neoplasms. tumors. oncology. including cancer and carcinogens cytology islam. bahai faith. theosophy, etc. philosophy. psychology. religion

Citations

No citations found. To add a citation, contact the admin at info@scimatic.org

Comments

No comments yet. Be the first to comment on this article.