Simultaneous electrochemical determination of ochratoxin A and fumonisin B1 with an aptasensor based on the use of a Y-shaped DNA structure on gold nanorods.

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ID: 84173
2020
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Abstract
A complementary DNA (cDNA) was designed to simultaneously hybridize with the ochratoxin A (OTA) aptamer and the fumonisin B1 (FB1) aptamer to form a unique Y-shaped DNA structure and to achieve simultaneous detection. Gold nanorods (AuNRs) were used to immobilize thionine (Th), thiolated ferrocene (Fc), thiolated OTA aptamer (Apt1), and thiolated FB1 aptamer (Apt2), to form an amplified signal element and a recognition element. The Apt1-AuNRs-Th complex and the Apt2-AuNRs-Fc complex hybridize with cDNA to form a unique Y-DNA structure on a gold electrode. This produces two initial electrochemical signals [with 177 μΑ cm near -0.2 V, and 3121 μΑ cm near +0.46 V (vs. Ag/AgCl)] by differential pulse voltammetry. Upon addition of 0.1 ng mL OTA and 0.1 ng mL FB1, the aptamers bind the two toxins. This results in the release of Apt1-AuNRs-Th and Apt2-AuNRs-Fc, so the peak currents densities decrease to 115 μΑ cm and 209 μΑ cm. The assay allows simultaneous determination of OTA and FB1 in the 1.0 pg·mL to 100 ng·mL concentration ranges, with LODs of 0.47 and 0.26 pg·mL. The assay is reproducible, stable and specific. It was applied to the determination of OTA and FB1 in spiked beer, with recoveries between 89.0% and 102.0%. Graphical abstractSchematic representation of OTA and FB detection based on Apt-AuNRs-Fc/Apt-AuNRs-Th/cDNA/AuE. (AuNRs: Gold nanorods; Th: thionine; Fc: ferrocene; SH: thiol; BSA: Bovine serum albumin; cDNA: Complementary DNA; Apt: Aptamer1; Apt: Aptamer2; OTA: Ochratoxin A; FB: Fumonisin B).
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wei2020simultaneousmikrochimica Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Wei, Min;Xin, Lingkun;Feng, Shuo;Liu, Yong;
Journal Mikrochimica acta
Year 2020
DOI
10.1007/s00604-019-4089-y
URL
Keywords
differential pulse voltammetry signal amplification multiplex detection fb1 aptamer-gold nanorods-thiolated ferrocene ota aptamer-gold nanorods-thionine

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