Distinguishing Anuran species by high-resolution melting analysis of the COI barcode (COI-HRM).

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ID: 84061
2019
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Abstract
Taxonomic identification can be difficult when two or more species appear morphologically similar. DNA barcoding based on the sequence of the mitochondrial cytochrome c oxidase 1 gene (COI) is now widely used in identifying animal species. High-resolution melting analysis (HRM) provides an alternative method for detecting sequence variations among amplicons without having to perform DNA sequencing. The purpose of this study was to determine whether HRM of the COI barcode can be used to distinguish animal species. Using anurans as a model, we found distinct COI melting profiles among three congeners of both spp. and spp. Sequence variations within species shifted the melting temperature of one or more melting domains slightly but do not affect the distinctness of the melting profiles for each species. An NMDS ordination plot comparing melting peak profiles among eight Anuran species showed overlapping profiles for and . The COI amplicon for both species contained two melting domains with melting temperatures that were similar between the two species. The two species belong to two different families, highlighting the fact that COI melting profiles do not reveal phylogenetic relationships but simply reflect DNA sequence differences among stretches of DNA within amplicons. This study suggests that high-resolution melting analysis of COI barcodes (COI-HRM) may be useful as a simple and rapid method to distinguish animal species that appear morphologically similar.
Reference Key
everman2019distinguishingecology Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Everman, Steven;Wang, Shiao Y;
Journal Ecology and evolution
Year 2019
DOI
10.1002/ece3.5808
URL
Keywords
species identification hrm coi dgge high‐resolution melting analysis

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