Data on the differentiation among () spp., () and () in Brazilian clinical samples using real-time PCR.

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ID: 84057
2020
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Abstract
This article contains the data regarding species identification in human and canine clinical samples from a Brazilian region endemic for () spp., () and () , using a previously developed approach involving two qPCR assays (qPCR-ML and qPCR-ama). The data are related to the article "Real-time PCR to differentiate among () subgenus, () and () : application on Brazilian clinical samples" [1], and include also details of clinical evaluation/diagnosis of human patients and primer sequences used in the qPCR assays. The species has been determined in 27 canine samples and 11 human samples, exploiting HRM analysis of qPCR-ML and Cq values of qPCR-ML and qPCR-ama, as reported previously [2]. The qPCR data were in agreement with the species characterization obtained with other methods such as conventional species-specific PCR, ITS1 PCR-RFLP or DNA sequencing. Despite the limited number of clinical samples, these data are encouraging for a potential application in regions where () spp., . (.) and . (.) are co-endemic.
Reference Key
diotallevi2020datadata Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Diotallevi, Aurora;Buffi, Gloria;Ceccarelli, Marcello;Neitzke-Abreu, Herintha Coeto;Gnutzmann, Laisa Vieira;da Costa Lima Junior, Manoel Sebastião;Di Domenico, Alice;De Santi, Mauro;Magnani, Mauro;Galluzzi, Luca;
Journal Data in brief
Year 2020
DOI
10.1016/j.dib.2019.104914
URL
Keywords
real-time pcr qpcr leishmania infantum hrm leishmania amazonensis viannia

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