Genetic variability and evolutionary implications of RNA silencing suppressor genes in RNA1 of sweet potato chlorotic stunt virus isolates infecting sweetpotato and related wild species.

侵染甘薯及其近缘野生种的甘薯褪绿矮化病毒分离株RNA1中RNA沉默抑制子基因的遗传变异及其进化意义

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ID: 83860
2013
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Abstract
BACKGROUND:The bipartite single-stranded RNA genome of Sweet potato chlorotic stunt virus (SPCSV, genus Crinivirus; Closteroviridae) encodes a Class 1 RNase III (RNase3), a putative hydrophobic protein (p7) and a 22-kDa protein (p22) from genes located in RNA1. RNase3 and p22 suppress RNA silencing, the basal antiviral defence mechanism in plants. RNase3 is sufficient to render sweetpotato (Ipomoea batatas) virus-susceptible and predisposes it to development of severe diseases following infection with unrelated virus. The incidence, strains and gene content of SPCSV infecting wild plant species have not been studied. METHODOLOGY/PRINCIPAL FINDINGS:Thirty SPCSV isolates were characterized from 10 wild Ipomoea species, Hewittia sublobata or Lepistemon owariensis (family Convolvulaceae) in Uganda and compared with 34 local SPCSV isolates infecting sweetpotatoes. All isolates belonged to the East African (EA) strain of SPCSV and contained RNase3 and p7, but p22 was not detected in six isolates. The three genes showed only limited genetic variability and the proteins were under purifying selection. SPCSV isolates lacking p22 synergized with Sweet potato feathery mottle virus (SPFMV, genus potyvirus; Potyviridae) and caused severe symptoms in co-infected sweetpotato plants. One SPCSV isolate enhanced accumulation of SPFMV, but no severe symptoms developed. A new whitefly-transmitted virus (KML33b) encoding an RNase3 homolog (<56% identity to SPCSV RNase3) able to suppresses sense-mediated RNA silencing was detected in I. sinensis. CONCLUSIONS/SIGNIFICANCE:SPCSV isolates infecting wild species and sweetpotato in Uganda were genetically undifferentiated, suggesting inter-species transmission of SPCSV. Most isolates in Uganda contained p22, unlike SPCSV isolates characterized from other countries and continents. Enhanced accumulation of SPFMV and increased disease severity were found to be uncoupled phenotypic outcomes of RNase3-mediated viral synergism in sweetpotato. A second virus encoding an RNase3-like RNA silencing suppressor was detected. Overall, results provided many novel and important insights into evolutionary biology of SPCSV.
背景：甘薯褪绿矮化病毒（Sweet potato chlorotic stunt virus, SPCSV；长线形病毒科 *Closteroviridae*，白粉虱传长线形病毒属 *Crinivirus*）的双分体单链RNA基因组中，RNA1编码1型RNase III（RNase3）、推定的疏水蛋白（p7）和22 kDa蛋白（p22）。RNase3和p22能够抑制植物的基础抗病毒防御机制——RNA沉默。RNase3足以使甘薯（*Ipomoea batatas*）对病毒易感，并在其感染无关病毒后诱发严重病害。此前，感染野生植物种类的SPCSV的发生率、株系及其基因组成尚未得到研究。 方法/主要发现：本研究对采集自乌干达10种野生番薯属植物、浅裂猪菜藤（*Hewittia sublobata*）或鳞蕊藤（*Lepistemon owariensis*）（均属于旋花科 Convolvulaceae）的30个SPCSV分离株进行了鉴定，并与感染甘薯的34个当地SPCSV分离株进行了比对分析。所有分离株均属于SPCSV东非（EA）株系并含有RNase3和p7基因，但在6个分离株中未检测到p22。这三个基因仅表现出有限的遗传变异性，且其编码蛋白处于纯化选择之下。缺失p22的SPCSV分离株仍能与甘薯羽状斑驳病毒（SPFMV；马铃薯Y病毒科 *Potyviridae*，马铃薯Y病毒属 *Potyvirus*）产生协同作用，导致共感染甘薯植株表现出严重症状。其中一个SPCSV分离株虽促进了SPFMV的积累，但植株未表现出严重症状。此外，在华番薯（*I. sinensis*）中检测到一种编码RNase3同源物（与SPCSV RNase3的一致性<56%）的新型粉虱传病毒（KML33b），该同源物能够抑制正义RNA介导的沉默。 结论/意义：乌干达境内感染野生植物与感染甘薯的SPCSV分离株在遗传上未发生明显分化，表明SPCSV存在种间传播。与在其他国家和大洲鉴定的SPCSV分离株不同，乌干达的大多数分离株均含有p22。研究发现，SPFMV积累量的增加与病害严重程度的加剧是甘薯中RNase3介导的病毒协同作用解偶联的表型表现。本研究还检测到了第二种编码RNase3样RNA沉默抑制子的病毒。总体而言，上述结果为SPCSV的进化生物学提供了诸多新颖且重要的见解。
Reference Key
tugume2013geneticplos Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Tugume, Arthur K;Amayo, Robert;Weinheimer, Isabel;Mukasa, Settumba B;Rubaihayo, Patrick R;Valkonen, Jari P T;
Journal PloS one
Year 2013
DOI
DOI not found
URL
http://europepmc.org/articles/PMC3838340?pdf=render
Keywords
Botany Medicine Science Agriculture (General)

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