Vascular Endothelial Growth Factor A and VEGFR-1 Change during Preimplantation in Heifers.

Клики: 312
ID: 81321
2020
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Аннотация
Vascular endothelial growth factor A (VEGFA) plays a critical angiogenic role in the endometrium of placentalia during preimplantation. The role of VEGFA and its receptors is not fully characterised in bovine reproduction. We analysed the mRNA expression of VEGFA isoforms 121, 165 and 189, and VEGF receptors 1 and 2 in three experimental settings (A, B and C). We compared intercaruncular endometrium of cyclic to pregnant heifers at Days 12, 15 and 18 post insemination (Day 0), and between Day 15 and Day 18 conceptuses (A). We further compared caruncular versus intercaruncular endometrium at Day 15 (B), and endometrium of heifers carrying embryos originating from somatic cell nuclear transfer (SCNT) versus in vitro fertilisation (IVF) at Day 18 (C). Endometrial VEGFA protein was localised and quantified. Pregnant heifers displayed lower intercaruncular endometrial mRNA expression of VEGFA-121 ( = 0.045) and VEGFA-189 ( = 0.009) as well as lower VEGFA protein abundance ( < 0.001) at Day 15. The VEGFA protein was localised in intercaruncular luminal, glandular epithelium and in tunica muscularis of blood vessels. At Day 15, caruncular endometrium displayed higher VEGFA mRNA expression than intercaruncular endometrium ( < 0.05). Intercaruncular endometrial VEGFA protein at Day 18 was higher in abundance in SCNT than in IVF ( = 0.038). Therefore, during preimplantation in cattle, there may be a need for timely physiological reduction in intercaruncular endometrial VEGFA expression in favour of the caruncular area to facilitate a gradient towards the implantation sites. A higher expression of VEGFA in SCNT may predispose for later placentation abnormalities frequently observed following SCNT.
Ссылочный ключ
chiumia2020vascularinternational Используйте этот ключ для автоцитирования в рукописи при использовании SciMatic Manuscript Manager или Thesis Manager
Авторы Chiumia, Daniel;Hankele, Anna-Katharina;Groebner, Anna E;Schulke, Katy;Reichenbach, Horst-Dieter;Giller, Katrin;Zakhartchenko, Valeri;Bauersachs, Stefan;Ulbrich, Susanne E;
Журнал International journal of molecular sciences
Год 2020
DOI
E544
URL
Ключевые слова
endometrium angiogenesis bovine ivf scnt conceptus

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