The Adaptive Morphology of Biofilms: A Defense Mechanism against Bacterial Starvation.

Clicks: 380
ID: 80079
2019
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Abstract
Biofilms are commonly defined as accumulations of microbes, embedded in a self-secreted, polysaccharide-rich extra-cellular matrix. This study aimed to characterize specific morphological changes that occur in biofilms under nutrient-limiting growth conditions. Under varying levels of nutrient depletion, colony-type biofilms were found to exhibit different rates of spatial expansion and green fluorescent protein production. Specifically, colony-type biofilms grown on media with decreased lysogeny broth content exhibited increased spatial expansion and more stable GFP production over the entire growth period. By modeling the surface morphology of colony-type biofilms using confocal and multiphoton microscopy, we analyzed the appearance of distinctive folds or "wrinkles" that form as a result of lysogeny broth content reduction in the solid agar growth media. When subjected to varying nutritional conditions, the channel-like folds were shown to alter their morphology; growth on nutrient-depleted media was found to trigger the formation of large and straight wrinkles connecting the colony core to its periphery. To test a possible functional role of the formed channels, a fluorescent analogue of glucose was used to demonstrate preferential native uptake of the molecules into the channels' interiors which supports their possible role in the transport of molecules throughout biofilm structures.
Reference Key
gingichashvili2019themicroorganisms Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Gingichashvili, Sarah;Duanis-Assaf, Danielle;Shemesh, Moshe;Featherstone, John D B;Feuerstein, Osnat;Steinberg, Doron;
Journal Microorganisms
Year 2019
DOI
E62
URL
Keywords
biofilm bacillus subtilis morphology stress response

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