Combined simultaneous enzymatic saccharification and comminution (SESC) and anaerobic digestion for sustainable biomethane generation from wood lignocellulose and the biochemical characterization of residual sludge solid.

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2019
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Abstract
Simultaneous enzymatic saccharification and comminution (SESC) was used for large-scale anaerobic digestion of wood lignocellulose to generate methane and unmodified lignin. During SESC, 10% aqueous mixture of powdered debarked wood from various species was subjected to bead milling with hydrolytic enzymes to generate particles below 1 μm. This slurry was directly used as a cosubstrate for anaerobic digestion in a 500 L stirred-tank reactor. Temperature and hydraulic retention time (HRT) were maintained at 50 °C and 30 days, respectively. At stable operation periods, an average yield of 224 L of methane per kg of cedar was attained. Comparable yields were achieved with red pine, elm, oak, and cedar bark. High-throughput microbial analysis established the presence of a relevant community to support the elevated level of methane production. The stability of the unmodified lignin in anaerobic digestion was also confirmed, allowing for its recovery as an important by-product.
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navarro2019combinedbioresource Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Navarro, Ronald R;Otsuka, Yuichiro;Matsuo, Kenji;Sasaki, Kei;Sasaki, Ken;Hori, Tomoyuki;Habe, Hiroshi;Nakamura, Masaya;Nakashimada, Yutaka;Kimbara, Kazuhide;Kato, Junichi;
Journal Bioresource technology
Year 2019
DOI
S0960-8524(19)31852-8
URL
Keywords
biomass lignocellulose microbial community anaerobic digestion methane gas sesc simultaneous enzymatic saccharification and comminution

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