Label-free electrochemical immunosensor based on enhanced signal amplification between Au@Pd and CoFe2O4/graphene nanohybrid.

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ID: 76644
2016
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Abstract
The improvement of sensitivity of electrochemical immunosensor can be achieved via two approaches: increasing loading capacities of antibody and enlarging responding electrochemical signals. Based on these, CoFe2O4/graphene nanohybrid (CoFe2O4/rGO) as support was firstly used for preparing electrochemical biosensor, and with the addition of Au@Pd nanorods (NRs) as mimic enzyme, a label-free electrochemical immunosensor was prepared. Due to the high electrical conductivity, open porous structure and large loading capacities of CoFe2O4/rGO, the enhanced signal amplification between Au@Pd NRs and CoFe2O4/rGO was studied. Fabricated as a novel substrate, the prepared immunosensor had a good analytical performance and exhibited a wide linear range from 0.01 to 18.0 ng · mL(-1) with a low detection limit of 3.3 pg · mL(-1) for estradiol, which was succeeded in applying to detect estradiol in the natural water.
Reference Key
zhang2016labelfreescientific Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Zhang, Yong;Li, Jiaojiao;Wang, Zhiling;Ma, Hongmin;Wu, Dan;Cheng, Qianhe;Wei, Qin;
Journal Scientific reports
Year 2016
DOI
10.1038/srep23391
URL
Keywords
electrochemical sensor estradiol diethylstilbestrol cu-btc frameworks environmental estrogens

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