In vitro germination and pollen tube growth of maize (Zea mays L.) pollen : VI. Combined effects of storage and the alleles at the waxy (wx), sugary (su 1)and shrunken (sh 2)loci.

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1972
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Abstract
Pollen grains containing either theWx,wx,Su 1,Su 1,Sh 2orsh 2alleles were stored for 0, 1, 2, 3, 4 or 5 days at 2 °C. After each storage period, a portion of each genotype was cultured on a 15% sucrose, 0.6% bacto-agar, 0.03% calcium nitrate and 0.01% boric acid medium, while another portion was placed on receptive silks, the number of kernels produced being a measure of fertilization ability. Regardless of the allele present in the pollen grain, 1 day of storage greatly increased the germination percentage and significantly increased pollen tube length. After 4 days of storage, there was noin vitro germination but some fertilization ability was found. The experiment was designed so that comparisons free from genetic background effects could be made between alleles at each locus. Significant differences at each storage period and a differential response to storage were obtained at some loci for germination percentage, ruptured percentage, pollen tube length and fertilization ability. A relationship between dominance of the allele and response to storage was detected only for fertilization ability. Since alleles at these loci affect the biochemical composition of pollen grains containing them, the results suggest that differences inin vitro germination characteristics and fertilization ability may be associated with biochemical composition.
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pfahler1972intag Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Pfahler, P L;Linskens, H F;
Journal tag theoretical and applied genetics theoretische und angewandte genetik
Year 1972
DOI
10.1007/BF00583416
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