Electrochemical micro analytical device interfaced with portable potentiostat for rapid detection of chlorpyrifos using acetylcholinesterase conjugated metal organic framework using Internet of things.

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ID: 75277
2019
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Abstract
An Electrochemical micro Analytical Device (EµAD) was fabricated for sensitive detection of organophosphate pesticide chlorpyrifos in the food chain. Gold microelectrode (µE) modified with Zinc based Metal Organic Framework (MOF-Basolite Z1200) and Acetylcholinesterase (AChE) enzyme served as an excellent electro-analytical transducer for the detection of chlorpyrifos. Electrochemical techniques such as Cyclic Voltammetry (CV), Electrochemical Impedance Spectroscopy (EIS) and Differential Pulse Voltammetry (DPV) were performed for electrochemical analysis of the developed EµAD. The sensor needs only 2 µL of the analyte and it was tested within the linear range of 10 to 100 ng/L. The developed EµAD's limit of detection (LoD) and sensitivity is 6 ng/L and 0.598 µ A/ng L/mm respectively. The applicability of the device for the detection of chlorpyrifos from the real vegetable sample was also tested within the range specified. The fabricated sensor showed good stability with a shelf-life of 20 days. The EµAD's response time is of 50 s, including an incubation time of 20 s. The developed EµAD was also integrated with commercially available low-cost, handheld potentiostat (k-Stat) using Bluetooth and the results were comparable with a standard electrochemical workstation.
Reference Key
nagabooshanam2019electrochemicalscientific Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Nagabooshanam, Shalini;Roy, Souradeep;Mathur, Ashish;Mukherjee, Irani;Krishnamurthy, Satheesh;Bharadwaj, Lalit M;
Journal Scientific reports
Year 2019
DOI
10.1038/s41598-019-56510-y
URL
Keywords
Machine learning mild cognitive impairment alzheimer’s disease data fusion bispectrum continuous wavelet transform

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