Effects of 17α-ethinylestradiol and bisphenol A on steroidogenic messenger ribonucleic acid levels in the rare minnow gonads.

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ID: 74042
2012
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Abstract
Previous studies showed that the endocrine disrupting chemicals (EDCs) affect reproductive physiology in teleosts. How the EDCs regulate gonadal steroidogenesis remains to be determined. The gonadal transcript changes of steroidogenic enzyme genes in adult rare minnow Gobiocypris rarus exposed to 17α-ethinylestradiol (EE2) and bisphenol A (BPA) were detected in the present study. The full-length cDNAs encoding steroidogenic enzymes, including steroidogenic acute regulatory protein (StAR), cytochrome P450-mediated side-chain cleavage enzyme (CYP11A1), 3β-hydroxysteroid dehydrogenase (3β-HSD), and cytochrome P450 17 A1 (CYP17A1) were isolated and characterized by RT-PCR and RACE methods. The homology and phylogenetic analyses of the amino acid sequences confirmed that the nucleotide sequences of these steroidogenic genes were correct. The mRNA tissue distribution results indicated that StAR, cyp11a1, and cyp17a1 mRNAs were mainly expressed in the gonads and 3β-HSD was mainly expressed in both the gonads and the brains. The 233 dpf adult G. rarus were exposed to EE2 (25ng/L) and BPA (5, 15, and 50 μg/L) dissolved in dimethyl sulfoxide (DMSO) or control for 7 days. The gonadal mRNA levels of StAR, cyp11a1, 3β-HSD, cyp17a1 and ovarian cytochrome P450 aromatase (cyp19a1a) were quantified by qRT-PCR. Our data indicated that 25 ng/L EE2 had different degrees of inhibitory effects on the expression of steroidogenic genes in the gonads. BPA at different levels caused concentration-specific effects on the mRNA expression of the steroidogenic genes. The transcripts of several ovarian steroidogenic genes were more sensitive to 15 μg/L BPA than that at other two levels. These findings suggest that EE2 could impair gonadal steroidogenesis by suppressing mRNA expression of steroidogenic genes and BPA could cause variations in gonadal steroidogenesis modulation with a potential consequence of compensation for the disturbance.
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Authors Liu, Shaozhen;Qin, Fang;Wang, Houpeng;Wu, Tingting;Zhang, Yingying;Zheng, Yao;Li, Meng;Wang, Zaizhao;
Journal Aquatic toxicology (Amsterdam, Netherlands)
Year 2012
DOI
10.1016/j.aquatox.2012.05.010
URL
Keywords
NMR HPLC sodium carboxymethyl cellulose SEM metabolic disease high-performance liquid chromatography gst ampk nox mtor traditional chinese medicine thin layer chromatography glutathione-s-transferase nf-κb mammalian target of rapamycin nuclear magnetic resonance dimethyl sulfoxide fcs pbs fetal calf serum peroxisome proliferator-activated receptor gamma tlc bovine serum albumin dmso bmp natural product bsa anova srebp pparγ dmem phosphate buffered saline lbd 3-isobutyl-1-methylxanthine amp-activated kinase atcc american type culture collection badge cmcna dulbecco's modified eagle's medium ec(50) egfp ibmx mef nadph-dependent oxidase nbs peroxisome proliferator-activated receptor rxr spf tcm tr-fret analysis of variance bisphenol a diglycidyl ether bone morphogenic protein 4 effective concentration 50% enhanced green fluorescent protein ligand-binding domain mouse embryonic fibroblasts newborn bovine serum nuclear factor κb retinoic x receptor specific pathogen free standard error of the mean sterol regulatory element binding protein time-resolved fluorescence resonance energy transfer

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