Development of a paper-based method to detect Hg in waste water using iturin from Bacillus subtilis.

Clicks: 293
ID: 73252
2019
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Abstract
Colorimetric, fluorescence, and paper-based method were developed to measure the Hg level in water using iturin A, a lipopeptide produced by Bacillus subtilis. Firstly, iturin was used to synthesize highly stable and uniformly sized silver nanoparticles (AgNPs). Secondly, the iturin-AgNPs were found to be highly selective and sensitive to Hg. The absorbance of the reaction system showed a good linear correlation with the Hg concentration from 0.5 to 5 mg/L at 450 nm in the UV-Vis spectroscopy detection with the limit of detection (LOD) of 0.5 mg/L. When the reaction system was detected by fluorescence measurement, a good linear relationship was found between the fluorescence intensity and Hg concentration from 0.05 to 0.5 mg/ at 415 nm with the LOD of 0.05 mg/L. Lastly, a paper-based detection method was developed. The developed method was successfully used to detect Hg in contaminated polluted waters and showed acceptable results in terms of sensitivity, selectivity and stability. The paper-based method could distinguish Hg at levels higher than 0.05 mg/L, thereby meeting the guidelines of the effluent quality standard for industries (0.05 mg/L). In summary, this method can be used daily by various industries to monitor the Hg level in effluent water.
Reference Key
zhao2019developmentapplied Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Zhao, Xixi;Ai, Chongyang;Li, Zhenzhu;Xu, Xiaoguang;Zhao, Haobin;Yan, Lu;Jiang, Chunmei;Shao, Dongyan;Shi, Junling;
Journal Applied microbiology and biotechnology
Year 2019
DOI
10.1007/s00253-019-10109-5
URL
Keywords
silver nanoparticle mercury ion biological synthesis fluorescence spectrophotometry test paper uv-visible spectrophotometry

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