A composite prepared from MoS quantum dots and silver nanoparticles and stimulated by mercury(II) is a robust oxidase mimetic for use in visual determination of cysteine.

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ID: 73249
2019
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Abstract
MoS quantum dots were hydrothermally synthesized and utilized for the formation and stabilization of a nanocomposite with silver nanoparticles (AgNPs) in a single step. This composite was characterized by transmission electron microscopy and zeta potential measurements. It is found that this nanohybrid can be stimulated by mercury(II) ion and then exhibits excellent oxidase mimicking activity. The oxidase-like activity is demonstrated by the oxidation of 3,3',5,5'-tetramethylbenzidine by HO that leads to the formation of a blue product. An assay was developed for determination of cysteine (Cys) at ultra-trace level because Cys inhibits the activity of the nanozyme via interaction with Hg(II). The Cys assay, best performed at a wavelength of 652 nm, works in the 1-100 μM concentration range and has a 0.82 μM detection limit. In addition, a portable Cys test kit is described that was applied to the determination of Cys in serum samples. The resulting colorations were compared with color chat wheel. The method is simple, rapid, cost-effective, and sensitive. Graphical abstractSchematic presentation of oxidase mimetic activity of the Hg@ MoS-QDs-AgNPs and colorimetric sensing of Cys.
Reference Key
ojha2019amikrochimica Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Ojha, Ravi Prakash;Mishra, Richa;Singh, Priya;Nirala, Narsingh Raw;Prakash, Rajiv;
Journal Mikrochimica acta
Year 2019
DOI
10.1007/s00604-019-4041-1
URL
Keywords
Silver nanoparticles colorimetry 3,3′,5,5′-tetramethylbenzidine l-cysteine assay mos2 quantum dots

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