Genomic resolution of a cold subsurface aquifer community provides metabolic insights for novel microbes adapted to high CO concentrations.

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ID: 72627
2017
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Abstract
As in many deep underground environments, the microbial communities in subsurface high-CO ecosystems remain relatively unexplored. Recent investigations based on single-gene assays revealed a remarkable variety of organisms from little studied phyla in Crystal Geyser (Utah, USA), a site where deeply sourced CO -saturated fluids are erupted at the surface. To provide genomic resolution of the metabolisms of these organisms, we used a novel metagenomic approach to recover 227 high-quality genomes from 150 microbial species affiliated with 46 different phylum-level lineages. Bacteria from two novel phylum-level lineages have the capacity for CO fixation. Analyses of carbon fixation pathways in all studied organisms revealed that the Wood-Ljungdahl pathway and the Calvin-Benson-Bassham Cycle occurred with the highest frequency, whereas the reverse TCA cycle was little used. We infer that this, and selection for form II RuBisCOs, are adaptions to high CO -concentrations. However, many autotrophs can also grow mixotrophically, a strategy that confers metabolic versatility. The assignment of 156 hydrogenases to 90 different organisms suggests that H is an important inter-species energy currency even under gaseous CO -saturation. Overall, metabolic analyses at the organism level provided insight into the biochemical cycles that support subsurface life under the extreme condition of CO saturation.
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probst2017genomicenvironmental Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Probst, Alexander J;Castelle, Cindy J;Singh, Andrea;Brown, Christopher T;Anantharaman, Karthik;Sharon, Itai;Hug, Laura A;Burstein, David;Emerson, Joanne B;Thomas, Brian C;Banfield, Jillian F;
Journal Environmental microbiology
Year 2017
DOI
10.1111/1462-2920.13362
URL
Keywords
carbon emission global supply chain reverse supply chain solid waste recycling

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