A molecular marker that is specific to medicinal rhubarb based on chloroplast trnL/trnF sequences.

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ID: 70903
2001
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Abstract
"Da-Huang" (Radix et Rhizoma Rhei, medicinal rhubarb), a famous and important Traditional Chinese Medicine, has often been confused with the adulterant species in the same genus, Rheum. Through sequencing the trnL (UAA)/trnF (GAA) regions of chloroplast DNA of thirteen species of Rheum (three medicinal rhubarb species and ten adulterant ones), a molecular marker of the medicinal species was found. A pair of PCR primers based on the sequences, was thus designed, which amplified a highly specific DNA fragment in medicinal rhubarb exclusively, and absent in the adulterants at all under an optimized PCR condition.
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Authors Yang, M;Zhang, D;Liu, J;Zheng, J;
Journal planta medica
Year 2001
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