Creation of Early Flowering Germplasm of Soybean by CRISPR/Cas9 Technology.

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ID: 70634
2019
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Ranked #31 of 187 articles by views in Frontiers in plant science

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Abstract
Soybean is an important economic crop and a typical short-day crop, sensitive to photoperiod, and has narrow geographical adaptative region, which limit the creation of transgenic materials and reduce the breeding efficiency of new varieties. In addition, the genetic transformation efficiency of soybean is lower than that of many other crops, and the available receptor genotypes are limited. In this study, -mediated transformation were used to introduce the CRISPR/Cas9 expression vector into soybean cultivar Jack and generated targeted mutants of gene controlling soybean flowering. We obtained two novel types of mutations, 11 bp and 40 bp deletion at coding region, respectively, and frameshift mutations produced premature translation termination codons and truncated E1 proteins, causing obvious early flowering under long day condition. In addition, no off-target effects were observed by predicting and analyzing the potential off-target sites of targets. Significant decreased gene expression of two novel mutants showed that the truncated E1 protein disinhibited / and increasing / gene expressions resulted obvious early flowering. Homozygous trans-clean mutants without T-DNA elements were also obtained and showed early flowering under long day condition. The photo-insensitive soybean transformation receptor we created laid a foundation for breeding excellent transgenic receptors suitable for high latitudes.
Reference Key
han2019creationfrontiers Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Han, Jianan;Guo, Bingfu;Guo, Yong;Zhang, Bo;Wang, Xiaobo;Qiu, Li-Juan;
Journal Frontiers in plant science
Year 2019
DOI
10.3389/fpls.2019.01446
URL
Keywords
soybean crispr/cas9 gene editing agrobacterium-mediated transformation e1

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