Improved operational stability of d-psicose 3-epimerase by a novel protein engineering strategy, and d-psicose production from fruit and vegetable residues.

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2016
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Abstract
The aim of the present work was to improve stability of d-psicose 3-epimerase and biotransformation of fruit and vegetable residues for d-psicose production. The study established that N-terminal fusion of a yeast homolog of SUMO protein - Smt3 - can confer elevated optimal temperature and improved operational stability to d-psicose 3-epimerase. The Smt3-d-psicose 3-epimerase conjugate system exhibited relatively better catalytic efficiency, and improved productivity in terms of space-time yields of about 8.5kgL(-1)day(-1). It could serve as a promising catalytic tool for the pilot scale production of the functional sugar, d-psicose. Furthermore, a novel approach for economical production of d-psicose was developed by enzymatic and microbial bioprocessing of fruit and vegetable residues, aimed at epimerization of in situd-fructose to d-psicose. The bioprocessing led to achievement of d-psicose production to the extent of 25-35% conversion (w/w) of d-fructose contained in the sample.
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Authors Patel, Satya Narayan;Sharma, Manisha;Lata, Kusum;Singh, Umesh;Kumar, Vinod;Sangwan, Rajender S;Singh, Sudhir P;
Journal Bioresource technology
Year 2016
DOI
10.1016/j.biortech.2016.05.053
URL
Keywords
d-psicose bioprocessing fruit and vegetable waste residual biomass smt3 d-psicose 3-epimerase

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