Modeling the bioconversion of polysaccharides in a continuous reactor: A case study of the production of oligogalacturonates by .

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2019
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Abstract
In the quest for a sustainable economy of the Earth's resources and for renewable sources of energy, a promising avenue is to exploit the vast quantity of polysaccharide molecules contained in green wastes. To that end, the decomposition of pectin appears to be an interesting target because this polymeric carbohydrate is abundant in many fruit pulps and soft vegetables. To quantitatively study this degradation process, here we designed a bioreactor that is continuously fed with de-esterified pectin (PGA). Thanks to the pectate lyases produced by bacteria cultivated in the vessel, the PGA is depolymerized into oligogalacturonates (UGA), which are continuously extracted from the tank. A mathematical model of our system predicted that the conversion efficiency of PGA into UGA increases in a range of coefficients of dilution until reaching an upper limit where the fraction of UGA that is extracted from the bioreactor is maximized. Results from experiments with a continuous reactor hosting a strain of the plant pathogenic bacterium and in which the dilution coefficients were varied quantitatively validated the predictions of our model. A further theoretical analysis of the system enabled an comparison of the efficiency of eight other pectate lyase-producing microorganisms with that of Our findings suggest that is the most efficient microorganism and therefore the best candidate for a practical implementation of our scheme for the bioproduction of UGA from PGA.
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sepulchre2019modelingthe Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Sepulchre, Jacques-Alexandre;Reverchon, Sylvie;Gouzé, Jean-Luc;Nasser, William;
Journal The Journal of biological chemistry
Year 2019
DOI
10.1074/jbc.RA118.004615
URL
Keywords
Microbiology enzyme kinetics pectin gene regulation mathematical modeling polysaccharide oligosaccharide continuous reactor dickeya dadantii dimensionless parameter galacturonate galacturonic acid pectate lyases unsaturated oligogalacturonate

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