Phenotypic and Genotypic Antimicrobial Resistance Traits of Non-O1/Non-O139 Isolated From a Large Austrian Lake Frequently Associated With Cases of Human Infection.

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ID: 70153
2019
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Abstract
belonging to serogroups other than O1 and O139 are opportunistic pathogens which cause infections with a variety of clinical symptoms. Due to the increasing number of non-O1/non-O139 infections in association with recreational waters in the past two decades, they have received increasing attention in recent literature and by public health authorities. Since the treatment of choice is the administration of antibiotics, we investigated the distribution of antimicrobial resistance properties in a non-O1/non-O139 population in a large Austrian lake intensively used for recreation and in epidemiologically linked clinical isolates. In total, 82 environmental isolates - selected on the basis of comprehensive phylogenetic information - and nine clinical isolates were analyzed for their phenotypic antimicrobial susceptibility. The genomes of 46 environmental and eight clinical strains were screened for known genetic antimicrobial resistance traits in CARD and ResFinder databases. In general, antimicrobial susceptibility of the investigated population was high. The environmental strains were susceptible against most of the 16 tested antibiotics, except sulfonamides (97.5% resistant strains), streptomycin (39% resistant) and ampicillin (20.7% resistant). Clinical isolates partly showed additional resistance to amoxicillin-clavulanic acid. Genome analysis showed that , a regulator of multidrug efflux genes, and the bicyclomycin/multidrug efflux system of were present in all isolates. Nine isolates additionally carried variants of and , determinants of beta-lactam resistance and six isolates carried , a determinant of phenicol resistance. Three isolates had both and . In 27 isolates, five out of six subfamilies of the MATE-family were present. For all isolates no genes conferring resistance to aminoglycosides, macrolides and sulfonamides were detected. The apparent lack of either known antimicrobial resistance traits or mobile genetic elements indicates that in cholera non-epidemic regions of the world, non-O1/non-O139 play a minor role as a reservoir of resistance in the environment. The discrepancies between the phenotypic and genome-based antimicrobial resistance assessment show that for non-O1/non-O139, resistance databases are currently inappropriate for an assessment of antimicrobial resistance. Continuous collection of both data over time may solve such discrepancies between genotype and phenotype in the future.
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lepuschitz2019phenotypicfrontiers Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Lepuschitz, Sarah;Baron, Sandrine;Larvor, Emeline;Granier, Sophie A;Pretzer, Carina;Mach, Robert L;Farnleitner, Andreas H;Ruppitsch, Werner;Pleininger, Sonja;Indra, Alexander;Kirschner, Alexander K T;
Journal Frontiers in microbiology
Year 2019
DOI
10.3389/fmicb.2019.02600
URL
Keywords
antibiotic resistance climate change vibrio cholerae bathing water non-o1/non-o139

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