Probing excited conformational states of nucleic acids by nitrogen CEST NMR spectroscopy.

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ID: 68294
2019
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Ranked #3 of 875 articles by views in journal of magnetic resonance (san diego, calif : 1997)

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Abstract
Characterizing low-populated and short-lived excited conformational states has become increasingly important for understanding mechanisms of RNA function. Interconversion between RNA ground and excited conformational states often involves base pairing rearrangements that lead to changes in the hydrogen-bond network. Here, we present two N chemical exchange saturation transfer (CEST) NMR experiments that utilize protonated and non-protonated nitrogens, which are key hydrogen-bond donors and acceptors, for characterizing excited conformational states in RNA. We demonstrated these approaches on the B. Cereus fluoride riboswitch, where N CEST profiles complement C CEST profiles in depicting a potential pathway for ligand-dependent allosteric regulation of the excited conformational state of the fluoride riboswitch.
Reference Key
zhao2019probingjournal Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Zhao, Bo;Baisden, Jared T;Zhang, Qi;
Journal journal of magnetic resonance (san diego, calif : 1997)
Year 2019
DOI
S1090-7807(19)30281-2
URL
Keywords
ligand binding chemical exchange rna dynamics riboswitch

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